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Single-cell Multiomics is an emerging technology that allows for parallel cell sorting and RNA sequencing operations. These instruments sort cells, via oligonucleotide barcoded tags, sorted into microwells, where cells are then retrieved for RNA sequencing, and the creation of detailed cell maps. Compared to traditional sequencing, single-cell multiomics instruments enhance distinctions of cellular heterogeneity within a sample.
The SeqPlex-I WTA kit allows amplification of small quantities of reverse transcribed RNA or degraded RNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2Step 1 In the Pre-amplification/Library Synthesis step using the (Library Preparation Reagents) the template RNA is reverse transcribed using primers composed of a semi-degenerate 3- and universal 5-ends. As polymerization proceeds displaced and RNaseH generated single strands serve as new templates for additional primer annealing and extension producing random overlapping cDNAs flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typicall
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The BD Rhapsody™ Express Single-Cell Analysis System is ideal for routine use with familiar cell systems that do not require visual QC or troubleshooting.
The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification kit enables unbiased three prime end capture, amplification and detection of transcriptomes at a single cell level.