Multiomics Instrumentation
Sigma Aldrich Fine Chemicals Biosciences SEQPLEX I WTA Kit Whole Tr
The SeqPlex-I WTA kit allows amplification of small quantities of reverse transcribed RNA or degraded RNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2Step 1 In the Pre-amplification/Library Synthesis step using the (Library Preparation Reagents) the template RNA is reverse transcribed using primers composed of a semi-degenerate 3- and universal 5-ends. As polymerization proceeds displaced and RNaseH generated single strands serve as new templates for additional primer annealing and extension producing random overlapping cDNAs flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typicall
AKOYA BIOSCIENCES FLOW CELLS FOR PHENOCYCLER-FUS
- Flow cells enable ultrahigh-plex spatial phenotyping and multiplex biomarker analysis.
- Compatible with the PhenoCycler-Fusion system for optimal performance.
- Facilitates single-cell resolution analysis on whole microscope slides.
- Designed for seamless integration with automated fluidics.
- Enables comprehensive biological insights by interrogating biomarkers in spatial context.
- Closed system design reduces contamination risks and user error.
- Ensures consistent and high-quality experimental outcomes.
- Supports 10 individual experiments for enhanced workflow efficiency.
- Shipping condition is ambient for straightforward storage and handling.
- Suitable for spatial phenotyping, multiplex biomarker analysis, and tissue analysis.
T-CELL TARGETED ANTIBODY- 1ML
- Targets CD3+ T cells
- Contains anti-human CD3 (OKT3) mAb
- Payload includes EGFP mRNA (m1Ψ)
- Utilizes ionizable lipid SM102
- Formulated with DSPC, PEG-2000 DMG, and cholesterol
- Stored in 10% sucrose in TBS
- Appearance is clear and colorless
- Concentration approximately 0.1 mg/mL
- Recommended storage at 4°C
- Stable for 6 months from manufacture date
- Designed for T cell research, immunotherapy, and cell tracking
- Research-grade quality
- Delivery via lipid nanoparticle system
- mRNA modified with N1-methyl-pseudouridine (m1Ψ)
- Covalent conjugation method
- Available as a premade format
- Ships with ice packs
- Customization options for targeting antibodies and formulations
BD Rhapsody™ Express
The BD Rhapsody™ Express Single-Cell Analysis System is ideal for routine use with familiar cell systems that do not require visual QC or troubleshooting.
BD Rhapsody™ Package
This instrument allows for single-cell mRNA capture upstream of library preparation.
BD™ Rhapsody™ Whole Transcriptome Analysis (WTA) Reagent Kit
The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification kit enables unbiased three prime end capture, amplification and detection of transcriptomes at a single cell level.
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